{"product_id":"biological-indicator-for-steam","title":"Biological Indicator for Steam","description":"\u003cdiv class=\"elementor-element elementor-element-7385ad13 elementor-align-center elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"7385ad13\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"elementor-widget-container\" data-mce-fragment=\"1\"\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"elementor-element elementor-element-5b997291 elementor-widget elementor-widget-woocommerce-product-additional-information\" data-id=\"5b997291\" data-element_type=\"widget\" data-widget_type=\"woocommerce-product-additional-information.default\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"elementor-widget-container\" data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_brand\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eBrand\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eBionova\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_process\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eProcess\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSteam\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_packaging\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePackaging\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e100\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_microorganism\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMicroorganism\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eGeobacillus Stearothermophilus (ATCC 7953)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_population\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePopulation\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e10^5, 10^6\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_read-out-time\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eRead-Out Time\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e24 h\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_conditions\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eConditions\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSee Product Description\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_regulations\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eRegulations\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIRAM 37102-1, IRAM 37102-3, ISO 11138-1, ISO 11138-3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eBT20 \/ Self-contained Biological Indicator for Steam sterilization processes.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eGeobacillus stearothermophilus\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e(ATCC 7953) 10^5 or 10^6 spores per vial. Readout: 24 hours.\u003c\/p\u003e\n\u003cp\u003eMonitoring vacuum-assisted and gravity air-displacement Steam Sterilization processes at 121-135 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInstructions for use\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Identify the Bionova® BT20 Indicator by writing the sterilizer number (in case of having  more than one), load number and processing date on the indicator label.\u003c\/p\u003e\n\u003cp\u003e2. Pack the biological indicator along with materials to be sterilized in an appropriate package according to recommended sterilization practices. Place this package in those areas which a priori you consider most inaccessible for the sterilizing agent (Steam). Generally, the center of the load and areas near the door are problematic.\u003c\/p\u003e\n\u003cp\u003e3. Sterilize as usual.\u003c\/p\u003e\n\u003cp\u003e4. After the sterilization process has finished, open the sterilizer door, wait for 5 minutes and remove the biological indicator from the package. CAUTION: Wear safety glasses and gloves when removing the Bionova® BT20 Biological Indicator from the sterilized package. WARNING: Do not crush or handle the biological indicator excessively, since this might cause the glass ampoule to burst.\u003c\/p\u003e\n\u003cp\u003e5. Allow the biological indicator to cool down to room temperature.\u003c\/p\u003e\n\u003cp\u003e6. Check the chemical indicator on the label of the biological indicator. A color change to brown confirms that the biological indicator has been exposed to steam. IMPORTANT: this color change does not indicate that the process was sufficient to achieve sterility. If the chemical indicator is unchanged, check the sterilization process.\u003c\/p\u003e\n\u003cp\u003e7. Crush the ampoule contained in the biological indicator with an individual ampoule crusher or with the ampoule crusher placed within the top of the incubator´s incubation area. Then shake the tube down vigorously, with movements similar to those performed to lower the temperature of a mercury thermometer, until the medium reaches the base of the tube and soaks the spore carrier entirely. Finally, place the biological indicator in the incubator.\u003c\/p\u003e\n\u003cp\u003eIMPORTANT: Use a non-sterilized biological indicator as a positive control at least once per day, when a sterilization cycle is run. The positive control ensures that appropriate incubation conditions were met. Both the positive control indicator and the processed indicator should belong to the same batch.\u003c\/p\u003e\n\u003cp\u003e8. Incubate the processed biological indicator and the indicator used as a positive control for a maximum of 24 hours between 55-62 ºC. Readout should be carried out at convenient intervals of 10 hours. Color change of the growth indicator medium from purple to yellow means that a sterilization process failure has occurred.\u003c\/p\u003e\n\u003cp\u003eIf at 24 hours there is no color change in the processed indicators, a final negative result is obtained (the sterilization process was effective). The positive control indicator should show a purple to yellow color change for the results to be valid. NOTE: To extend the incubation time beyond 24 hours, we recommend using a humidified environment to prevent complete evaporation of the culture medium contained within the indicator.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Bionova","offers":[{"title":"24 Hr Biological Indicator | 100\/Pk","offer_id":47452117368884,"sku":"BIO-BT20","price":199.99,"currency_code":"CAD","in_stock":true},{"title":"Super Fast Self-Contained - 3 hr Readout | 50\/ PK","offer_id":47452117401652,"sku":"BIO-BT220","price":309.99,"currency_code":"CAD","in_stock":true},{"title":"Super Fast Self-Contained - 1 hr Readout | 50\/Pk","offer_id":47452117434420,"sku":"BIO-BT222","price":329.99,"currency_code":"CAD","in_stock":true},{"title":"Ultra Fast Self-Contained Biological Indicator. - 20 Min | 50\/PK","offer_id":47452117467188,"sku":"BIO-BT224","price":499.99,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0746\/9776\/9012\/files\/0_9335138c-23de-401f-9e68-32d39f4f3cb0.jpg?v=1769505925","url":"https:\/\/onestopdental.ca\/products\/biological-indicator-for-steam","provider":"One Stop Dental Supply","version":"1.0","type":"link"}